||Human query sequences of less than 100 amino acids (sequence length limitation for accurate gene tree estimation). Example.
||No hits in BLAST search from teleost genomes with E-value cut-off of < 10^(-3). Example.
|(iii) 1,874: No clear orthologs identified between tetrapods and teleosts in the estimated gene tree.
||No clear orthogroups identified. Example.
||Excessively long branch lengths (tentatively ≥2.0 base substitution per site) between tetrapods and teleosts. Example.
||Less than 4 sequences remained after long branch deletion process (limitation of # of sequences for phylogenetic analysis). Example.
||Less than 4 Blast hits. Example.
||Limited length of unambiguously aligned sites for accurate gene tree estimation. Example.
|(v) 702: Failure in BLAST search or amino acid translation due to erroneous sequence data.
||cDNA and amino acid sequence was not match in DB. ExampleExample.
||Erroneous amino acid sequence unavailable for Blast search. Example.